Overview of Available Biological Samples
Donated biological samples were provided near the time of enrolment or during a subsequent sample collection campaign. Biosamples available for access include serum, plasma, DNA, red blood cells, cryo-preserved whole blood and urine samples. Time to last meal was recorded at the time of blood donation. Urine samples were collected at random day time. If a participant was unable to provide a blood sample due to medical or geographical reasons, a saliva sample or blood spot was sought whenever possible.
Biological Sample Collection
All blood samples were collected from participants via arm venipuncture into Becton Dickinson vacutainer tubes as per manufacture instructions. Serum was collected in a SST tube. Plasma, buffy coat and red blood cells were collected in a K2EDTA tube. In a subset of participants, PST-plasma and ACD whole blood were collected.
Urine, when requested, was collected in a sterile cup without preservatives. Saliva samples were collected, often at the participant’s home, in an Oragene™ Saliva kit. Blood spots were collected at the participant’s home using a Whatman® 903 card.
Biological Sample Shipping
For blood and urine samples processed and aliquoted onsite at the phlebotomy collection location, samples were shipped and transported frozen at -80oC. For those phlebotomy collection sites without onsite biological sample aliquoting services, biological samples were shipped and transported in batches to be received by the regional processing lab often the same day or the next day. Spun-SST, spun-PST, EDTA and urine samples were shipped on ice while ACD samples were shipped and transported at ambient temperature.
Saliva and blood spot samples were shipped at ambient temperature.
Biological Sample Processing
Full biosample processing details are available upon request. Biosample pre-analytical history is available for each biosample in the form of the Standard Preanalytical Coding for Biospecimens (SPREC)(Biopreservation and Biobanking; 2012:10(4) 366-374) and Biospecimen Reporting for Improved Study Quality (BRISQ) (Cancer Cytopathology, 2011;119:92-101).
Long Term Biological Sample Storage
Blood and urine samples are stored at -80oC or at -190oC. DNA is stored at -20oC or colder. Oragene™ saliva samples are stored at ambient temperature as per the manufacturer’s suggested storage.
Legacy Biological Samples
A portion of each participant’s biological sample is reserved for Legacy. Studies requiring access to Legacy biological samples will require further evaluation as they represent the last available amount for a given biological sample. Such studies must be unable to use other biological samples within the biobank and are of exceptional scientific merit and cannot be answered using another readily available resource.
Applying for Biological Sample Access
Researchers interested in accessing CPTP biosamples are encouraged to review the biosample guidelines and feasibility review criteria, as well as to contact the Access Office with any questions prior to completing and submitting the CPTP Data and Material Access Application Form.
Biological Sample Release to Approved Users
Most biological samples were initially stored in large volumes to reduce the required amount of freezer space. Biological samples will be thawed and sub-aliquoted prior to release to approved users. Biological samples will be released in 0.5 mL ThermoMatrix® tubes placed in ThermoMatrix® racks (8X12 format). DNA samples can also be supplied in plates upon request.
Pre-analytical variables related to biological sample collection, shipping, processing and storage will be provided with the released data. Data supplied will include the SPREC code and BRISQ variables.
Biological Sample Shipment to Approved Users
Biological samples will be shipped, to approved users at their designated laboratory, when ready. Only couriers that meet CPTP shipping requirements will be selected for service. Scheduling arrangements will be made prior to shipping. Upon receipt of biological samples, the approved user is required to confirm their receipt within 24 hours.